Department of Medicine


Intravital Core Highlighted Publications

Ferrell, N.; Sandoval, R. M.; Molitoris, B. A.; Brakeman, P.; Roy, S.; Fissell, W. H., Application of physiological shear stress to renal tubular epithelial cells. Methods Cell Biol 2019, 153, 43-67.

Molitoris, B. A., DNA damage response protects against progressive kidney disease. The Journal of clinical investigation 2019, 129 (11), 4574-4575.

Molitoris, B. A., Beyond Biomarkers: Machine Learning in Diagnosing Acute Kidney Injury. Mayo Clinic proceedings 2019, 94 (5), 748-750.

Molitoris, B. A., Rethinking CKD Evaluation: Should We Be Quantifying Basal or Stimulated GFR to Maximize Precision and Sensitivity? American journal of kidney diseases : the official journal of the National Kidney Foundation 2017, 69 (5), 675-683.

Molitoris, B. A.; George, A. G.; Murray, P. T.; Meier, D.; Reilly, E. S.; Barreto, E.; Sandoval, R. M.; Rizk, D. V.; Shaw, A. D.; Peacock, W. F., A Novel Fluorescent Clinical Method to Rapidly Quantify Plasma Volume. Cardiorenal Med 2019, 9 (3), 168-179.

Sandoval, R. M.; Molitoris, B. A.; Palygin, O., Fluorescent Imaging and Microscopy for Dynamic Processes in Rats. Methods in molecular biology (Clifton, N.J.) 2019, 2018, 151-175.

Uchida, M.; Maier, B.; Waghwani, H. K.; Selivanovitch, E.; Pay, S. L.; Avera, J.; Yun, E.; Sandoval, R. M.; Molitoris, B. A.; Zollman, A.; Douglas, T.; Hato, T., The archaeal Dps nanocage targets kidney proximal tubules via glomerular filtration. The Journal of clinical investigation 2019, 129 (9), 3941-3951.

Bayliss, G. P.; Cobb, J.; Decker, B.; Hellman, R.; Vasavada, N.; Mackelaite, L.; Shadur, C.; Ilori, T.; Ibrahim, T.; Leight, K.; Hsiao, L. L.; Molitoris, B. A.; Okusa, M. D.; Parker, M. G., Kidney Mentoring and Assessment Program for Students: a guide for engaging medical students in nephrology. Clin Kidney J 2019, 12 (6), 761-766.

Liu, X. F.; Wei, J.; Zhou, Q.; Molitoris, B. A.; Sandoval, R.; Kobayashi, H.; Okada, R.; Nagaya, T.; Karim, B.; Butcher, D.; Pastan, I., Immunotoxin SS1P is rapidly removed by proximal tubule cells of kidney, whose damage contributes to albumin loss in urine. Proc Natl Acad Sci U S A 2020, 117 (11), 6086-6091.

Kolb AL, et al. (2018) Exogenous Gene Transmission of Isocitrate Dehydrogenase 2 Mimics Ischemic Preconditioning Protection. J Am Soc Nephrol. (4):1154-1164

Sandoval RM, Molitoris BA. (2017) Intravital multiphoton microscopy as a tool for studying renal physiology and pathophysiology. Methods. 128:20-32. 

Endres BT, et al. (2017) Intravital imaging of the kidney in a rat model of salt-sensitive hypertension. Am J Physiol Renal Physiol. (2):F163-F173

Hato T, et al.(2017) Two-photon intravital fluorescence lifetime imaging of the kidney reveals cell-type specific metabolic signatures. J Am Soc NephrolIn Process.

Day RN, Tao W, & Dunn KW (2016) A simple approach for measuring FRET in fluorescent biosensors using two-photon microscopy. Nat Protoc 11(11):2066-2080.

Hato T, et al. (2016) Novel application of complimentary imaging techniques to examine in vivo glucose metabolism in the kidney. Am J Physiol Renal Physiol:ajprenal 00535 02015.

Wagner MC, et al. (2016) Proximal Tubules Have the Capacity to Regulate Uptake of Albumin. J Am Soc Nephrol 27(2):482-494.

Wagner MC, et al. (2016) Mechanism of increased clearance of glycated albumin by proximal tubule cells. Am J Physiol Renal Physiol 310(10):F1089-1102.

Tao W, et al. (2015) A practical method for monitoring FRET-based biosensors in living animals using two-photon microscopy. Am J Physiol Cell Physiol 309(11):C724-735.

El-Achkar TM & Dagher PC (2015) Tubular cross talk in acute kidney injury: a story of sense and sensibility. Am J Physiol Renal Physiol 308(12):F1317-1323.

Hato T & Dagher PC (2015) How the Innate Immune System Senses Trouble and Causes Trouble. Clin J Am Soc Nephrol 10(8):1459-1469.

Hato T, et al. (2015) The macrophage mediates the renoprotective effects of endotoxin preconditioning. J Am Soc Nephrol 26(6):1347-1362.

Hato T, Sandoval R, & Dagher PC (2015) The caspase 3 sensor Phiphilux G2D2 is activated non-specifically in S1 renal proximal tubules. Intravital 4(2).

Ferrell N, et al. (2015) Shear stress is normalized in glomerular capillaries following (5/6) nephrectomy. Am J Physiol Renal Physiol 308(6):F588-593.

Ryan JC, Dunn KW, & Decker BS (2014) Effects of chronic kidney disease on liver transport: quantitative intravital microscopy of fluorescein transport in the rat liver. Am J Physiol Regul Integr Comp Physiol 307(12):R1488-1492.

Sandoval RM, Wang E, & Molitoris BA (2014) Finding the bottom and using it: Offsets and sensitivity in the detection of low intensity values in vivo with 2-photon microscopy. Intravital 2(1).

Dickson LE, Wagner MC, Sandoval RM, & Molitoris BA (2014) The proximal tubule and albuminuria: really! J Am Soc Nephrol 25(3):443-453.

Carrisoza-Gaytan R, et al. (2014) Effects of biomechanical forces on signaling in the cortical collecting duct (CCD). Am J Physiol Renal Physiol 307(2):F195-204.

Molitoris BA (2014) Therapeutic translation in acute kidney injury: the epithelial/endothelial axis. J Clin Invest 124(6):2355-2363.

Hall AM & Molitoris BA (2014) Dynamic multiphoton microscopy: focusing light on acute kidney injury. Physiology (Bethesda) 29(5):334-342.

Hato T, El-Achkar TM, & Dagher PC (2013) Sisters in arms: myeloid and tubular epithelial cells shape renal innate immunity. Am J Physiol Renal Physiol 304(10):F1243-1251.

Hall AM, Rhodes GJ, Sandoval RM, Corridon PR, & Molitoris BA (2013) In vivo multiphoton imaging of mitochondrial structure and function during acute kidney injury. Kidney Int 83(1):72-83.

Sandoval RM & Molitoris BA (2013) Quantifying glomerular permeability of fluorescent macromolecules using 2-photon microscopy in Munich Wistar rats. J Vis Exp (74).

Molitoris BA & Sandoval RM (2009) Techniques to study nephron function: microscopy and imaging. Pflugers Arch 458(1):203-209.

Dunn KW, Sutton TA, & Sandoval RM (2012) Live-animal imaging of renal function by multiphoton microscopy. Curr Protoc Cytom Chapter 14:Unit12 19.

Babbey CM, et al. (2012) Quantitative intravital microscopy of hepatic transport. Intravital 1:1-10.

Wang E, et al. (2012) A portable fiberoptic ratiometric fluorescence analyzer provides rapid point-of-care determination of glomerular filtration rate in large animals. Kidney Int 81(1):112-117.

Sandoval RM, et al. (2012) Multiple factors influence glomerular albumin permeability in rats. J Am Soc Nephrol 23(3):447-457.

Imamura R, et al. (2012) A nonerythropoietic derivative of erythropoietin inhibits tubulointerstitial fibrosis in remnant kidney. Clin Exp Nephrol 16(6):852-862.

Saleh MA, et al. (2012) Chronic endothelin-1 infusion elevates glomerular sieving coefficient and proximal tubular albumin reuptake in the rat. Life Sci 91(13-14):634-637.

Thompson JD, et al. (2012) Toxicological and pharmacokinetic properties of chemically modified siRNAs targeting p53 RNA following intravenous administration. Nucleic Acid Ther 22(4):255-264.

Choong FX, Sandoval RM, Molitoris BA, & Richter-Dahlfors A (2012) Multiphoton microscopy applied for real-time intravital imaging of bacterial infections in vivo. Methods Enzymol 506:35-61.

Presson RG, Jr., et al. (2011) Two-photon imaging within the murine thorax without respiratory and cardiac motion artifact. Am J Pathol 179(1):75-82.

Kalakeche R, et al. (2011) Endotoxin uptake b     by S1 proximal tubular segment causes oxidative stress in the downstream S2 segment. J Am Soc Nephrol 22(8):1505-1516.

Basile DP, et al. (2011) Impaired endothelial proliferation and mesenchymal transition contribute to vascular rarefaction following acute kidney injury. Am J Physiol Renal Physiol 300(3):F721-733.

Melican K, et al. (2011) Uropathogenic Escherichia coli P and Type 1 fimbriae act in synergy in a living host to facilitate renal colonization leading to nephron obstruction. PLoS Pathog 7(2):e1001298.

Sharfuddin AA & Molitoris BA (2011) Pathophysiology of ischemic acute kidney injury. Nat Rev Nephrol 7(4):189-200.

Molitoris BA & Sandoval RM (2011) Kidney endothelial dysfunction: ischemia, localized infections and sepsis. Contrib Nephrol 174:108-118.

Kamocka MM, et al. (2010) Two-photon intravital imaging of thrombus development. J Biomed Opt 15(1):016020.

Wang E, Sandoval RM, Campos SB, & Molitoris BA (2010) Rapid diagnosis and quantification of acute kidney injury using fluorescent ratio-metric determination of glomerular filtration rate in the rat. Am J Physiol Renal Physiol 299(5):F1048-1055.

Imamura R, et al. (2010) Intravital two-photon microscopy assessment of renal protection efficacy of siRNA for p53 in experimental rat kidney transplantation models. Cell Transplant 19(12):1659-1670.

Molitoris BA & Sandoval RM (2010) Multiphoton imaging techniques in acute kidney injury. Contrib Nephrol 165:46-53.

Campos SB, et al. (2009) Cytokine-induced F-actin reorganization in endothelial cells involves RhoA activation. Am J Physiol Renal Physiol 296(3):F487-495.

Sharfuddin AA, et al. (2009) Soluble thrombomodulin protects ischemic kidneys. J Am Soc Nephrol 20(3):524-534.

Russo LM, et al. (2009) Impaired tubular uptake explains albuminuria in early diabetic nephropathy. J Am Soc Nephrol 20(3):489-494.

Molitoris BA, et al. (2009) siRNA targeted to p53 attenuates ischemic and cisplatin-induced acute kidney injury. J Am Soc Nephrol 20(8):1754-1764.

Gupta A, Williams MD, Macias WL, Molitoris BA, & Grinnell BW (2009) Activated protein C and acute kidney injury: Selective targeting of PAR-1. Curr Drug Targets 10(12):1212-1226.

Dunn KW & Sutton TA (2008) Functional studies in living animals using multiphoton microscopy. ILAR J 49(1):66-77.

Melican K, et al. (2008) Bacterial infection-mediated mucosal signalling induces local renal ischaemia as a defence against sepsis. Cell Microbiol 10(10):1987-1998.

Wagner MC, et al. (2008) Ischemic injury to kidney induces glomerular podocyte effacement and dissociation of slit diaphragm proteins Neph1 and ZO-1. J Biol Chem 283(51):35579-35589.

Sandoval RM & Molitoris BA (2008) Quantifying endocytosis in vivo using intravital two-photon microscopy. Methods Mol Biol 440:389-402.

Sharfuddin AA, Sandoval RM, & Molitoris BA (2008) Imaging techniques in acute kidney injury. Nephron Clin Pract 109(4):c198-204.

El-Achkar TM, Plotkin Z, Marcic B, & Dagher PC (2007) Sepsis induces an increase in thick ascending limb Cox-2 that is TLR4 dependent. Am J Physiol Renal Physiol 293(4):F1187-1196.

Mansson LE, et al. (2007) Real-time studies of the progression of bacterial infections and immediate tissue responses in live animals. Cell Microbiol 9(2):413-424.

Horbelt M, et al. (2007) Organic cation transport in the rat kidney in vivo visualized by time-resolved two-photon microscopy. Kidney Int 72(4):422-429.

Gupta A, et al. (2007) Activated protein C ameliorates LPS-induced acute kidney injury and downregulates renal INOS and angiotensin 2. Am J Physiol Renal Physiol 293(1):F245-254.

Yu W, Sandoval RM, & Molitoris BA (2007) Rapid determination of renal filtration function using an optical ratiometric imaging approach. Am J Physiol Renal Physiol 292(6):F1873-1880.

Russo LM, et al. (2007) The normal kidney filters nephrotic levels of albumin retrieved by proximal tubule cells: retrieval is disrupted in nephrotic states. Kidney Int 71(6):504-513.

Ashworth SL, Sandoval RM, Tanner GA, & Molitoris BA (2007) Two-photon microscopy: visualization of kidney dynamics. Kidney Int 72(4):416-421.

Molitoris BA & Sandoval RM (2007) Quantifying dynamic kidney processes utilizing multi-photon microscopy. Contrib Nephrol 156:227-235.

Mansson LE, Melican K, Molitoris BA, & Richter-Dahlfors A (2007) Progression of bacterial infections studied in real time--novel perspectives provided by multiphoton microscopy. Cell Microbiol 9(10):2334-2343.

Wagner MC, Molnar EE, Molitoris BA, & Goebl MG (2006) Loss of the homotypic fusion and vacuole protein sorting or golgi-associated retrograde protein vesicle tethering complexes results in gentamicin sensitivity in the yeast Saccharomyces cerevisiae. Antimicrob Agents Chemother 50(2):587-595.

Suurna MV, et al. (2006) Cofilin mediates ATP depletion-induced endothelial cell actin alterations. Am J Physiol Renal Physiol 290(6):F1398-1407.

Sandoval RM, et al. (2006) A non-nephrotoxic gentamicin congener that retains antimicrobial efficacy. J Am Soc Nephrol 17(10):2697-2705.

Molitoris BA & Sandoval RM (2006) Pharmacophotonics: utilizing multi-photon microscopy to quantify drug delivery and intracellular trafficking in the kidney. Adv Drug Deliv Rev 58(7):809-823.

Herget-Rosenthal S & Molitoris BA (2006) Advances in imaging the kidney and cellular processes. Nephron Exp Nephrol 103(2):e31-32.

Molitoris BA & Sandoval RM (2005) Intravital multiphoton microscopy of dynamic renal processes. Am J Physiol Renal Physiol 288(6):F1084-1089.

Yu W, Sandoval RM, & Molitoris BA (2005) Quantitative intravital microscopy using a Generalized Polarity concept for kidney studies. Am J Physiol Cell Physiol 289(5):C1197-1208.

Tanner GA, Sandoval RM, Molitoris BA, Bamburg JR, & Ashworth SL (2005) Micropuncture gene delivery and intravital two-photon visualization of protein expression in rat kidney. Am J Physiol Renal Physiol 289(3):F638-643.

Tanner GA, Sandoval RM, & Dunn KW (2004) Two-photon in vivo microscopy of sulfonefluorescein secretion in normal and cystic rat kidneys. Am J Physiol Renal Physiol 286(1):F152-160.

Atkinson SJ, Hosford MA, & Molitoris BA (2004) Mechanism of actin polymerization in cellular ATP depletion. J Biol Chem 279(7):5194-5199.

Sandoval RM & Molitoris BA (2003) Gentamicin traffics retrograde through the secretory pathway and is released in the cytosol via the endoplasmic reticulum. Am J Physiol Renal Physiol 286(4):F617-624.

Ashworth SL, et al. (2004) Renal ischemia induces tropomyosin dissociation-destabilizing microvilli microfilaments. Am J Physiol Renal Physiol 286(5):F988-996.

Sandoval RM, Kennedy MD, Low PS, & Molitoris BA (2004) Uptake and trafficking of fluorescent conjugates of folic acid in intact kidney determined using intravital two-photon microscopy. Am J Physiol Cell Physiol 287(2):C517-526.

Molitoris BA & Sutton TA (2004) Endothelial injury and dysfunction: role in the extension phase of acute renal failure. Kidney Int 66(2):496-499.

Kelly KJ, Sandoval RM, Dunn KW, Molitoris BA, & Dagher PC (2003) A novel method to determine specificity and sensitivity of the TUNEL reaction in the quantitation of apoptosis. Am J Physiol Cell Physiol 284(5):C1309-1318.

Dunn KW, Sandoval RM, & Molitoris BA (2003) Intravital imaging of the kidney using multiparameter multiphoton microscopy. Nephron Exp Nephrol 94(1):e7-11.

Ashworth SL, et al. (2003) ADF/cofilin mediates actin cytoskeletal alterations in LLC-PK cells during ATP depletion. Am J Physiol Renal Physiol 284(4):F852-862.

Sutton TA, et al. (2003) Injury of the renal microvascular endothelium alters barrier function after ischemia. Am J Physiol Renal Physiol 285(2):F191-198.

Dunn KW, et al. (2002) Functional studies of the kidney of living animals using multicolor two-photon microscopy. Am J Physiol Cell Physiol 283(3):C905-916.